Isolation of the skin progenitor cells in canine allergic dermatitis and their application for the pathogenesis

2012 Fiscal Year Final Research Report

• Project/Area Number: 23780330
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Clinical veterinary science
• Research Institution: Azabu University
• Principal Investigator: KAWARAI Shinpei 麻布大学, 大学病院, 助教 (20533079)
• Project Period (FY): 2011 – 2012
• Keywords: 皮膚前駆細胞 / アレルギー性皮膚炎 / 犬 / 創傷治癒 / 骨髄造血幹細胞

Research Abstract

Role of the skin progenitor cells in the pathogenesis of allergic dermatitis (AD) was evaluated by using dogs, known to spontaneously develop the disease. We found CD34-positive skin progenitor cells were localized in the skin lesion of canine AD. An isolation method of CD34-positive hematopoietic progenitor cells was developed for the in vitro studies. Our results and established methods could be useful for the further research of the mechanisms of skin progenitor cells in the allergic inflammation.

Research Products

• [Journal Article] Validation of New Bite-Block-Type Head-Immobilization Devices for Radiotherapy in Dogs (2013)
  • Author(s): Maruo T, Nakamura S, Fukuyama Y, Kawarai S
  • Journal Title: Veterinary Radiology & Ultrasound Volume: (in press)
  • Peer Reviewed
• [Journal Article] Identification of Cell Surface Antigen Expression in Canine (2013)
  • Author(s): Fujimoto A, Neo S, Ishizuka C, Kato T, Segawa K, Kawarai S, Ogihara K, Hisasue M, Tsuchiya R.
  • Journal Title: Hepatocellular Carcinoma Cell Lines Volume: (in press)
  • Peer Reviewed
• [Journal Article] A case of cutaneous sterile pyogranuloma/ granuloma syndrome in a Maltese (2013)
  • Author(s): Kawarai, S., Matsuura, S., Yamamoto, S., Kiuchi, A., Kanemaki, N., Madarame, H., Shirota, K
  • Journal Title: J Am Anim Hosp Assoc Volume: (in press)
  • Peer Reviewed
• [Presentation] VTのための犬のアレルギー性皮膚炎をもつ飼い主さんへの豆知識 (2013)
  • Author(s): 川原井晋平
  • Organizer: 日本臨床獣医学フォーラム九州地区大会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 20130300
• [Presentation] 犬のアレルギー性皮膚炎の診断・治療入門編 (2013)
  • Author(s): 川原井晋平
  • Organizer: 第9回日本獣医内科学アカデミー学術大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 20130200
• [Presentation] 外因性グルココルチコイドのコルチゾールと甲状腺ホルモン分泌能に対する影響 (2012)
  • Author(s): 山本園子、川原井晋平、茅沼秀樹、久末正晴、土屋亮
  • Organizer: 第87回麻布獣医学会
  • Place of Presentation: 麻布大学(神奈川)
  • Year and Date: 20121100
• [Presentation] 国産コナヒョウヒダニ抗原液の有用性に関する再考 (2012)
  • Author(s): 細川聖矢、川原井晋平、津久井利広、久末正晴、印牧信行、斑目広郎、土屋亮、小方宗次
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120900
• [Presentation] 外因性グルココルチコイドの副腎機能と甲状腺機能に対する影響(臨床と実験的検討) (2012)
  • Author(s): 佐藤穂高、川原井晋平、茅沼秀樹、根尾櫻子、伊藤哲郎、久末正晴、土屋亮
  • Organizer: 第43回日本比較臨床医学会
  • Place of Presentation: 専修大学(神奈川)
  • Year and Date: 20120700
• [Presentation] 2次元ポリアクリルミドゲル電気泳動法による犬血清中抗βH-クリスタリン自己抗体の検出 (2012)
  • Author(s): 印牧信行、川原井晋平、高橋弘樹、仁科有美子、市川陽一朗
  • Organizer: 第153回獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティ(埼玉)
  • Year and Date: 20120300
• [Presentation] 犬のアレルギー性皮膚炎の症状維持におけるマイクロバブルを用いたシャンプー療法の有効性の検討 (2012)
  • Author(s): 飯野瑞貴、西本優子、藤本あゆみ、野澤源太、川原井晋平、信田卓男、木内明男
  • Organizer: 日本動物看護学会関西地区第5回例会
  • Place of Presentation: 大阪府立大学(大阪)
  • Year and Date: 20120300
• [Presentation] 犬における無菌性肉芽腫性皮膚炎の一例 (2011)
  • Author(s): 松浦祐介、高橋亮二、川原井晋平、山口忠義、斑目広郎、代田欣二
  • Organizer: 関東・東京合同地区獣医師大会
  • Place of Presentation: 日本大学生物資源科学部(神奈川)
  • Year and Date: 20110900
• [Presentation] A case of granulomatous dermatitis in a dog (2011)
  • Author(s): Kawarai S, Takahashi R, Madarame H, Shirota K
  • Organizer: The Asian Meeting of Animal MedicineSpecialties
  • Place of Presentation: IMPACT ConventionCenter (Thailand)
  • Year and Date: 20110500
• [Book] アレルギーわんこの暮らしとレシピ百科 (2011)
  • Author(s): 小方宗次監修,斑目広郎,川原井晋平
  • Publisher: 誠文堂新光社