Molecular analysis of SH3P2 function during cell migration

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: TANIMURA Susumu 長崎大学, 大学院・医歯薬学総合研究科, 助教 (90343342)
• Project Period (FY): 2011 – 2012
• Keywords: 癌 / シグナル伝達 / 細胞運動 / リン酸化 / MAPキナーゼ

Research Abstract

SH3P2, a novel regulator of cell migration, binds to hnRNP-K and Myosin1E. Overexpression of SH3P2 inhibited the splicing of MMP-3/-9 mRNA. GST pull down/LC-MS/MS assay revealed that SH3 domain of Myosin1E interacts with Caveolin1-Cavin complex, which plays important roles in caveolae formation. These results indicate that SH3P2 regulates cell migration via hnRNP-K-mediated mRNA splicing of MMPs and Myosin1E-mediated caveolae formation

Research Products

• [Journal Article] Blockade of the ERK pathway enhances the therapeutic efficacy of the histone deacetylase inhibitor MS-275 in human tumor xenograft models (2013)
  • Author(s): Sakamoto, T., Ozaki, K., Fujio, K., Kajikawa, S., Uesato, S., Watanabe, K., Tanimura, S., Koji, K., Kohno, M
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 13 Pages: 00406-3
  • DOI: doi:10.1016/j.bbrc.2013.03.009
  • Peer Reviewed
• [Journal Article] Central role of the exchange factor GEF-H1 in TNF-α-induced sequential activation of Rac, ADAM17/TACE, and RhoA in tubular epithelial cells (2013)
  • Author(s): Waheed, F., Dan, Q., Amoozadeh, Y., Zhang, Y., Tanimura, S., Speight, P., Kapus, A., Szaszi, K
  • Journal Title: Mol. Biol. Cell Volume: 24 Pages: 1068-1082
  • DOI: doi:10.1091/mbc.E12-09-0661
  • Peer Reviewed
• [Journal Article] Functional characterization of the novel BRAF complex mutation, BRAF^, identified in papillary thyroid carcinoma (2013)
  • Author(s): Matsuse, M., Mitsutake, N., Tanimura, S., Ogi,T., Nishihara, E., Hirokawa, M., Fuziwara,CS., Saenko, VA., Suzuki, K., Miyauchi, A., Yamashita, S
  • Journal Title: Int. J. Cancer Volume: 132 Pages: 738-743
  • DOI: doi:10.1002/ijc.27709
  • Peer Reviewed
• [Journal Article] Up-regulation of pro-apoptotic protein Bim and down-regulation of anti-apoptotic protein Mcl-1 cooperatively mediate enhanced tumor cell death induced by the combination of ERK kinase (MEK) inhibitor and microtubule inhibitor (2012)
  • Author(s): Kawabata, T.,Tanimura, S., Asai, K., Kawasaki, R., Matsumaru, Y., Kohno, M
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 10289-10300
  • DOI: doi:10.1074/jbc.M111.319426.
  • Peer Reviewed
• [Journal Article] New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl group. Bioorg (2011)
  • Author(s): Kawaratani, Y., Harada, T., Hirata, Y., Nagaoka, Y., Tanimura, S., Shibano, M., Taniguchi, M., Yasuda, M., Baba, K., Uesato, S
  • Journal Title: Med. Chem Volume: 19 Pages: 3995-4003
  • DOI: doi:10.1016/j.bmc.2011.05.031
  • Peer Reviewed
• [Journal Article] Targeting the extracellular signal-regulated kinase pathway in cancer therapy (2011)
  • Author(s): Kohno, M., Tanimura, S., Ozaki, K
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 1781-1784
• [Presentation] Myosin1Eの細胞運動亢進作用はUNC45-Hsp90複合体による細胞内局在制御によって調節される (2012)
  • Author(s): 谷村進
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2012-12-15
• [Presentation] UNC45によるMyosin1Eの細胞内局在制御 (2012)
  • Author(s): 平田弦也
  • Organizer: 第29回日本薬学会九州支部大会
  • Place of Presentation: 熊本大学薬学部
  • Year and Date: 2012-12-09
• [Presentation] Myosin1Eによるカベオラ形成制御と細胞運動 (2012)
  • Author(s): 中原康子
  • Organizer: 第29回 日本薬学会九州支部大会
  • Place of Presentation: 熊本大学薬学部
  • Year and Date: 2012-12-09
• [Presentation] MEK and microtubule inhibitors together kill tumor cells through up-regulation of Bim and down-regulation of Mcl-1 (2012)
  • Author(s): 谷村進
  • Organizer: 第71回日本癌学会学術大会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-20
• [Presentation] Myosin1Eによる細胞運動制御の分子メカニズム (2011)
  • Author(s): 森田和幹
  • Organizer: 第28回日本薬学会九州支部大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2011-12-10
• [Presentation] Myosin1E enhances cell motility via the induction of filopodia formation: essential role of phosphorylation on Ser736 (2011)
  • Author(s): 谷村進
  • Organizer: 第70回日本癌学会学術大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-05