2012 Fiscal Year Final Research Report
Function of cyclophilin A and CD147 in rheumatoid arthritis
| Project/Area Number |
23790311
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | CypA / CD147 / 関節リウマチ |
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| Research Abstract |
Cyclophilin A (CypA), a member of the immunophilin family, is a ubiquitously distributed intracellular protein. Recent studies have shown that CypA is secreted by cells in response to inflammatory stimuli. Elevated levels of extracellular CypA and its receptor, CD147 have been detected in the synovium of patients with RA. However, the precise process of interaction between CypA and CD147 in the development of RA remains unclear. This study aimed to investigate CypA secretion from fibroblast-like synoviocytes (FLS) isolated from mice with collagen-induced arthritis (CIA) and CypA-induced CD147 expression in mouse macrophages. Both CypA and CD147 were highly expressed in the joints of CIA mice. CD147 was expressed in the infiltrated macrophages in the synovium of CIA mice. In vitro, spontaneous CypA secretion from FLS was detected and this secretion was increased by stimulation with LPS. CypA markedly increased CD147 levels in macrophages. These findings suggest that an interaction in the synovial joints between extracellular CypA and CD147 expressed by macrophages forms one of the critical mechanisms underlying RA pathogenesis.
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[Journal Article] Brain pericytes among cells constituting the blood-brain barrier are highly sensitive to tumor necrosis factor-α, releasing matrix metalloproteinase-9 and migrating in vitro.2011
Author(s)
Takata F, Dohgu S, Matsumoto J, Takahashi H, Machida T, Wakigawa T, Harada E,Miyaji H, Koga M, Nishioku T, Yamauchi A, Kataoka Y.
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Journal Title
J Neuroinflammation
Volume: 8
Pages: 106
DOI
Peer Reviewed
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