2013 Fiscal Year Final Research Report
Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients
Project/Area Number |
23790886
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Circulatory organs internal medicine
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Research Institution | Kurume University |
Principal Investigator |
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Project Period (FY) |
2011 – 2013
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Keywords | 血管新生細胞 / 血小板由来膜小胞体 / RANTES / 血管新生 |
Research Abstract |
We investigated whether PMPs could augment the neovascularization. We isolated mononuclear cells and PMPs from atherosclerotic patient-derived peripheral blood and generated PMP-pretreated CACs(PMP-CACs) by co-culture of the mononuclear cells and PMPs. The adhesion capacity of PMP-CACs was greater to that of CACs. PMPs released RANTES into the culture medium. Intravenous injection of PMP-CACs to rats with hindlimb ischemia augmented neovascularization of the ischemic limbs more than the injection of CACs.The number of PMP-CACs incorporated into the capillaries of the ischemic limbs was greater than that of incorporated CACs.The augmented adhesion and neovascularization capacities by PMP-CACs were canceled out by a RANTES neutralizing antibody. PMP-secreted RANTES may play role in the augmenting adhesion and neovascularization capacities of CACs. Injection of PMP-CACs may be a new strategy to augment the effects of therapeutic angiogenesis for limb ischemia in atherosclerotic patients.
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Research Products
(2 results)