Analysis of the molecular mechanism of radiation induced invasiveness

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Hokkaido University
• Principal Investigator: NAM JinMin 北海道大学, 大学院・医学研究科, 特任助教 (60414132)
• Research Collaborator: ONODERA Yasuhito 北海道大学, 大学院・医学研究科, 助教 (90435561)
• Project Period (FY): 2011 – 2012
• Keywords: 放射線 / 乳癌 / 浸潤 / インテグリン

Research Abstract

Radiation therapy is the common treatment for breast cancer, and the primary goal of therapy is to prevent invasive recurrence. The detailed molecular mechanism of invasive recurrence after radiation therapy is not well understood. This study investigated the effect of radiation on a three-dimensional culture model of non-invasive breast cancer, and identified important molecule and possible molecular mechanism underlying emergence of invasiveness. Our results suggest that α5ss1-integrin via NF-κB signaling is an important mediator of invasive progression after radiation treatment on non-invasive breast cancer cells in three-dimensional extracellular matrix.

Research Products

• [Journal Article] ss1-integrin via NF- κ B signaling is essential for acquisition of invasiveness in a model of radiation treated in situ breast cancer. (2013)
  • Author(s): Nam JM, Ahmed KM, Costes S, Zhang H, Onodera Y, Olshen AB, Hatanaka KC, Kinoshita R, Ishikawa M, Sabe H, Shirato H, Park CC
  • Journal Title: Breast Cancer Res
  • Peer Reviewed
• [Journal Article] Rab5c promotes AMAP1-PRKD2 complex formation to enhance ss 1 integrin recycling in EGF-induced cancer invasion. (2012)
  • Author(s): Onodera Y, Nam JM, Hashimoto A, Norman JC, Shirato H, Hashimoto S, Sabe H
  • Journal Title: J Cell Biol Volume: 25;197(7) Pages: 983-96
  • DOI: doi:10.1083/jcb.201201065.
  • Peer Reviewed
• [Journal Article] Intracellular trafficking of integrins in cancer cells.
  • Author(s): Onodera Y, Nam JM, Sabe H
  • Journal Title: Pharmacol Ther
  • DOI: doi:10.1016/j.pharmthera.2013.05.007.
  • Peer Reviewed
• [Presentation] Targeting beta1 integrin suppresses invasive recurrence in a three-dimensional model of radiation treated ductal carcinoma in situ (2012)
  • Author(s): Nam JM, Ahmed KM, Costes S, Zhang H, Sabe H, Shirato H, Park CC
  • Organizer: ASTRO 54th Annual meeting
  • Place of Presentation: Boston Convention and Exhibition Center (USA)
  • Year and Date: 20121028-31
• [Presentation] Co-overexpression of GEP100 With EGFR correlates with early recurrence after breast conservation therapy (BCT) (2012)
  • Author(s): Kinoshita R, Nam JM, Hosoda M, Kubota KC, Tanino M, Hashimoto A, Ito YM, Tanaka S, Sabe H, Shirato H
  • Organizer: ASTRO 54th Annual meeting
  • Place of Presentation: Boston Convention andExhibition Center(USA)
  • Year and Date: 20121028-31
• [Presentation] 非浸潤性乳管癌の3次元培養細胞モデルにおける放射線の影響と再発に関 わる分子機序の解析 (2012)
  • Author(s): 南ジンミン ,Ahmed Kazi, CostesSylvain, Zhang Hui, 佐邊壽孝, 白土博樹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌・さっぽろ芸文館・札幌市教育文化会館(北海道)
  • Year and Date: 20120919-21
• [Presentation] 癌浸潤におけるAMAP1-PRKD2複合体によるインテグリンリサイクリングとその制御機構 (2012)
  • Author(s): 小野寺康仁、南ジンミン、橋本あり、白土博樹、橋本茂、佐邊壽孝
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌・さっぽろ芸文館・札幌市教育文化会館(北海道)
  • Year and Date: 20120919-21
• [Presentation] Inhibition of integrin suppresses invasive recurrence in a 3D model of radiation treated DCIS (2012)
  • Author(s): Nam JM, Ahmed KM, Costes S, Zhang H, Sabe H, Shirato H, Park CC
  • Organizer: The 10th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: Hokkaido University (Sapporo)
  • Year and Date: 2012-10-03
• [Presentation] Targeting integrin signaling to suppress invasive recurrence post- ionizing radiation in a 3D cell culture model of ductal carcinoma in situ (2011)
  • Author(s): Nam JM, Zhang H, Sabe H, Shirato H,Park CC
  • Organizer: AACR specific conference: Advances in breast cancer research
  • Place of Presentation: The Fairmont SanFrancisco (USA)
  • Year and Date: 20111012-15
• [Presentation] Coordinate upregulation of α5ss1-integrin and fibronectin by malignant breast cells in three dimensional cultures can be specifically targeted to enhance radiotherapy (2011)
  • Author(s): Nam JM, Onodera Y, Shirato H, Bissell MJ, Park CC
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 20110627-29
• [Presentation] Targeting integrin signaling to suppress invasive recurrence post- ionizing radiation in a 3D cell culture model of ductal carcinoma insitu (2011)
  • Author(s): Nam JM, Zhang H, Sabe H, Shirato H, Park CC
  • Organizer: The 9th Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: Hokkaido University (Sapporo)
  • Year and Date: 2011-09-30