Development of anti cancer drug targeting longevity related gene -SIRT1 using nanotechnology.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791811
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokyo
• Principal Investigator: SONE Kembun 東京大学, 医学部付属病院, 助教 (90598872)
• Project Period (FY): 2011 – 2012
• Keywords: SIRT1 / DBC-1 / アポトーシス / カスパーゼ / E-cadherin / 子宮内膜 / 子宮体癌 / 細胞接着能

Research Abstract

We elucidated function analysis of SIRT1 and DBC-1 in apoptosis. And We analyzed the function of SIRT1 in endometrium.
We confirmed that DBC-1 is targeted for cleavage by caspase-7 during the process of apoptosis. In addition, Endgeneous SIRT1 and DBC-1 localize to the nucleus in healthy cells and to the cytoplasm during apoptosis, and possibly induce apoptosis signal.
In endometrium,SIRT1 plays an important role in regulating E-cadherin and induce cell-attachment.and There is possiblility that SIRT1 is related to human cancinogene- sis in endometrium and capacity of an embrio to attach.

Research Products

• [Journal Article] equential effects of the proteasome inhibitor bortezomib and chemotherapeutic agents in uterine cervical cancer cell lines (2012)
  • Author(s): Miyamoto Y, Nakagawa S, Wada-Hiraike O, Seiki T, Tanikawa M, Hiraike H, Sone K, Nagasaka K, Oda K, Kawana K, Nakagawa K, Fujii T, Yano T, Kozuma S, Taketani Y.
  • Journal Title: Oncology Reports Volume: 29 Pages: 51-57
  • DOI: DOI:0.3892/or.2012.2072.
  • Peer Reviewed
• [Journal Article] Regulation of SIRT1 determines initial step of endometrial receptivity by controlling E-caderine expression (2012)
  • Author(s): Akira Shirane, Osamu Wada-Hiraike,Mic hihiro Tanikawa,Takayuki Seiki,Haruko H iraike,Yuichiro Miyamoto,Kenbun Sone et al
  • Journal Title: Biochemical and Biophysical Research Communication Volume: 424 Pages: 604-610
  • DOI: DOI:10.1016/j.bbrc.2012.06.160
  • Peer Reviewed
• [Journal Article] Genotype-dependent efficacy of a dual PI3K/mTOR inhibitor, NVP-BEZ235, and an mTOR inhibitor, RAD001, in endometrial carcinomas (2012)
  • Author(s): Shoji K, Oda K, Kashiyama T, Ikeda Y, Nakagawa S, Sone K, Miyamoto Y, Hiraike H, Tanikawa M, Miyasaka A, Koso T, Matsumoto Y, Wada-Hiraike O, Kawana K, Kuramoto H, McCormick F, Aburatani H, Yano T, Kozuma S, Taketani Y.
  • Journal Title: PLoS One Volume: 7(5)
  • DOI: dio:10.1371/journal.pone.0037431
  • Peer Reviewed
• [Journal Article] Resveratrol promotes expression on SIRT1 And StAR in rad ovarian granulosa cells (2012)
  • Author(s): Morita Y,Wada-Hiraike O,Yano T,Shirane A,Hirano M,Hiraike H,Koyama S,Oisihi H, Yoshino O,Miyamoto Y,Sone K,Oda K, Nakagawa S,Tsutsui k,Taketani Y
  • Journal Title: an Implicative role of SIRT1 in the ovary Reproductive Biology and endocrinology Volume: 10 Pages: 14
  • DOI: DOI:11,1186/1477-7287-10-14
  • Peer Reviewed
• [Journal Article] Multifunctional transpription factor TF II?1 is an activator of BRACA1 function (2011)
  • Author(s): Tanikawa M,Wada-Hiraike O,Nakagawa S, Shirane A,Hiraike H,Koyama S,Miyamoto Y Sone K,Tsuruga T,Nagasaka K,Matsumoto Y, Ikeda Y,Shoji K,Oda K,Fukuhara H,Nakagawa K,Kato S,Yano T,Taketani Y
  • Journal Title: British journal of cancer Volume: 104 Pages: 1349-1355
  • DOI: DOI:10.1038/bjc2011.75
  • Peer Reviewed
• [Presentation] 子宮体癌リンパ節転移陽性(臨床進行期IIIc)例における予後因子の検討 (2012)
  • Author(s): 園田正樹、織田克利,有本貴英,武家尾舞子,曾根献文,森繭代,松本陽子,川名敬,中川俊介,矢野哲,上妻志郎,武谷雄二
  • Organizer: 日本婦人科腫瘍学会
  • Place of Presentation: 新高輪プリンスホテル
  • Year and Date: 2012-07-19
• [Presentation] 抗老化分子SIRT1活性化物 (2012)
  • Author(s): 白根晃他
  • Organizer: 日本内分泌学会
  • Place of Presentation: 名古
  • Year and Date: 2012-04-19
• [Presentation] 再発卵巣癌に対するsalvage chemotherapy (2011)
  • Author(s): 曾根献文、松本陽子、森繭代、有本貴英、
  • Organizer: 日本癌治療学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-27
• [Presentation] 当科での子宮内膜異型増殖症、高分化子宮体癌に対するMPA療法を用いた妊孕性温存治療に関する検討胚細胞性腫瘍に対するBEP療法の有害事象と妊孕性に関する検討 (2011)
  • Author(s): 宮本雄一郎、佐藤英貴,三浦紫保,長阪一憲,曾根献文,松本陽子,有本貴英,織田克利,川名敬,矢野哲,上妻志郎,武谷雄二
  • Organizer: 日本婦人科腫瘍学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-07-22
• [Presentation] 胚細胞性腫瘍に対するBEP療法の有害事象 (2011)
  • Author(s): 曾根献文、有本貴英、川名敬、塚崎雄大、
  • Organizer: 日本婦人科腫瘍学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-07-22