2012 Fiscal Year Final Research Report
Role of Sox9 transcriptional network during chondrocyte differentiation
| Project/Area Number |
23792121
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
HATA Kenji 大阪大学, 歯学研究科, 准教授 (80444496)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA Riko 大阪大学, 歯学研究科, 教授 (60294112)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 軟骨細胞 / Sox9 / 転写因子 |
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| Research Abstract |
Sox9 is a transcription factor critical for endochondral ossification; however, proof of its epigenetic regulation remains elusive. Here, we identified Arid5b (AT-rich interactive domain 5b) as a novel transcriptional co-regulator for Sox9. Arid5b physically interacted with Sox9 and cooperatively promoted chondrogenesis through direct binding to the Col2a1gene promoter. Arid5b^-/-mice showed growth retardation with delayed chondrogenesis. The methylation level of H3K9 in chondrogenic marker gene promoters was markedly increased in Arid5b^-/- chondrocytes. In conclusion, our findings establish a novel epigenomic mechanism of skeletal development, where Arid5b promotes chondrogenesis by facilitating a relationship between Sox9 and Phf2-mediated histone demethylation of chondrogenic gene promoters.
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Research Products
(14 results)
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[Journal Article] Spatiotemporal disorder in the axial skeleton development of theMesp2-null mouse: a model of spondylocostal dysostosis and spondylothoracicdysostosis2013
Author(s)
Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K,Kanno J, Yoneda T.Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A,Iimura T
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Journal Title
Bone
Volume: 53(1)
Pages: 248-58
Peer Reviewed
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[Journal Article] Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification2012
Author(s)
Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G, Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T
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Journal Title
J Biol Chem
Volume: 287(40)
Pages: 33179-90
Peer Reviewed
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[Journal Article] Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription2011
Author(s)
Amano K, Hata K, Muramatsu S, Wakabayashi M, Takigawa Y, Ono K, Nakanishi M,Takashima R, Kogo M, Matsuda A, Nishimura R, Yoneda T
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Journal Title
Mol Biol Cell
Volume: 22(8)
Pages: 1300-11
Peer Reviewed
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