Effects of enamel matrix protein on the metabolism of periodontal tissue and application of enamel matrix protein for orthodontic treatment.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792428
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KUNIMATSU Ryo 広島大学, 病院, 歯科診療医 (40580915)
• Project Period (FY): 2011 – 2012
• Keywords: 蛋白質 / 生理活性 / シグナル伝達 / 歯学 / 再生歯学

Research Abstract

It is demonstrated that human full-length amelogenin enhances the proliferation of human cementoblasts lineage cells by an interaction with CD63 through MAPK-ERK signaling pathway. In addition, amelogenin up-regulated the expressions of bone specific marker and mineralization activity in the osteogenic differentiation of human cementoblasts. In vivo studies,it is demonstrated that application of amelogenin surely suppresses root resorption. It is thus confirmed that amelogenin may contributeto the regeneration of cementum in terms of the enhancement of human cementoblast lineage cells proliferation and differentiation, indicating an application of amelogenin to regeneration of periodontal tissue.

Research Products

• [Journal Article] Amelogenin Enhances the Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Bone Marrow (2012)
  • Author(s): Tanimoto K, Huang YC, Tanne Y, Kunimatsu R, Michida M, Yoshioka M, Ozaki N, Sasamoto T, Yoshimi Y, Kato Y, Tanne K
  • Journal Title: Cells Tissues Organs Volume: 196(5) Pages: 411-9
  • Peer Reviewed
• [Journal Article] Differential effects of amelogenin on mineralization of cementoblasts and periodontal ligament cells (2012)
  • Author(s): Tanimoto K, Kunimatsu R, Tanne Y, Huang YC, Michida M, Yoshimi Y, Miyauchi M, Takata T, Tanne K
  • Journal Title: J Periodontol Volume: 83(5) Pages: 672-9
  • Peer Reviewed
• [Journal Article] Amelogenin enhances the proliferation of cementoblast lineage cells (2012)
  • Author(s): Kunimatsu R, Tanimoto K, Tanne Y, Kamiya T, Ohkuma S, Huang YC, Yoshimi Y, Miyauchi M, Takata T, Tanne K
  • Journal Title: J Periodontol Volume: 82(11) Pages: 1632-8
  • Peer Reviewed
• [Journal Article] 矯正歯科における初期う蝕への先進的対応 バイオミネラリゼーション技術によるハイドロキシアパタイトの結晶形成 (2012)
  • Author(s): 谷本幸太郎、國松 亮、丹根一夫
  • Journal Title: 広大歯誌 Volume: 44(2) Pages: 112-122
  • Peer Reviewed
• [Presentation] Amelogenin variants promote the proliferation and diffentiation of human cemetoblasts (2013)
  • Author(s): Yoshimi Y, Tanimoto K, Kunimatsu, R, Hirose N,Awada T, Mitsuyoshi T,Tanne K
  • Organizer: The 112thAmerican association of orthodontics
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20130503-07
• [Presentation] エナメル蛋白アメロゲニンのC末端側がヒトセメント芽細胞の増殖・分化に及ぼす影響 (2012)
  • Author(s): 吉見有希、谷本幸太郎、國松 亮、廣瀬尚人、粟田哲也、丹根由起、吉岡基子、光吉智美、鷲見圭輔、蘇 少卿、沖 奈苗、岡本友希、高田 隆、丹根一夫
  • Organizer: 第71回日本矯正歯科学術大会
  • Place of Presentation: 盛岡
  • Year and Date: 20120926-28
• [Presentation] エナメル芽細胞の増殖および分化に対するアメロブラスチンの生理活性 (2011)
  • Author(s): 廣瀬尚人, 島津 篤, 谷本幸太郎, 丹根由起, 國松 亮, 尾崎徳継, 吉見友希, 内田 隆, 丹根一夫
  • Organizer: 第70回日本矯正歯科学術大会・第4回国際会議
  • Place of Presentation: 名古屋
  • Year and Date: 20111117-20
• [Presentation] バイオミネラリゼーション技術を応用したエナメル質再生法-むし歯進行予防のための新たな戦略 (2011)
  • Author(s): 國松 亮, 谷本幸太郎, 丹根由起, 神谷貴志, 岩渕泰憲, 吉岡基子, 尾崎徳継, 廣瀬尚人, 笹本智子, 道田将彦, 光吉智美, 吉見友希, 鷲見圭輔, 蘇 少卿, 丹根一夫
  • Organizer: 第70回日本矯正歯科学術大会・第4回国際会議
  • Place of Presentation: 名古屋
  • Year and Date: 20111117-20
• [Presentation] Differential effects of amelogenin on mineralization of periodontal cells (2011)
  • Author(s): Yoshimi Y, Tanimoto K, Kunimatsu R, Takata T, Tanne K
  • Organizer: The 59th annual meeting of Japanese association for dental research
  • Place of Presentation: Hiroshima
  • Year and Date: 20111008-09
• [Presentation] Amelogenin-guided Biomineralization of Hydroxyapatite Crystal on Tooth Enamel (2011)
  • Author(s): Tanimoto K, Kunimatsu R,Yoshimi Y, Yoshioka M, Tanne Y, Tanne K
  • Organizer: 4th Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 20111008-09
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/orthod/orthod-j.html
• [Patent(Industrial Property Rights)] ヒトアメロゲニンペプチドとその生理活性を応用した薬剤 (2012)
  • Inventor(s): 谷本幸太郎, 丹根一夫, 國松 亮, 吉見友希
  • Industrial Property Rights Holder: 国立大学法人広島大学
  • Industrial Property Number: 特願2012-016734
  • Filing Date: 2012-01-30
• [Patent(Industrial Property Rights)] エナメル質再生液及びエナメル質再生キット (2011)
  • Inventor(s): 谷本幸太郎, 丹根一夫, 國松 亮, 神谷貴志.
  • Industrial Property Rights Holder: 国立大学法人広島大学
  • Industrial Property Number: 特願2011-027923
  • Filing Date: 2011-02-10