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2012 Fiscal Year Final Research Report

New practical technique for Protein Crystallization with Mebiol Gel

Research Project

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Project/Area Number 23860028
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied materials science/Crystal engineering
Research InstitutionOsaka University

Principal Investigator

SUGIYAMA Shigeru  大阪大学, 理学(系)研究科(研究院), 特任准教授 (90615428)

Project Period (FY) 2011 – 2012
Keywordsメビオールゲル / アガロースゲル / 固相ゲル中結晶化 / タンパク質結晶化 / X線構造解析 / 創薬 / 分子設計
Research Abstract

In this study, we successfully crystallized three proteins in Mebiol gel. Our analysis indicates that Mebiol gel provides new possibilities for overcoming the inherent problem of high-temperature damage to the proteins. We also investigated the nucleation rate of protein crystals in the presence of Mebiol gel and found that Mebiol gel increased the number of protein crystals. Furthermore, the Mebiol gel surrounding the crystals reduces the influence of osmotic shock in high-ionic-strength solutions. This technique will open up a new dimension for the crystallization of biological macromolecules in hydrogels.

  • Research Products

    (13 results)

All 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] A novel approach for protein crystallization by a synthetic hydrogel with thermoreversible gelation polymer2013

    • Author(s)
      S. Sugiyama, N. Shimizu, G. Sazaki, M. Hirose, Y. Takahashi, M. Maruyama, H. Matsumura, H. Adachi, K. Takano, S. Murakami, T. Inoue, and Y. Mori
    • Journal Title

      Cryst. Growth Des.

      Volume: 13 Pages: 1899-1904

    • Peer Reviewed
  • [Journal Article] Effects of Gel-Solution Interfaces on Femtosecond Laser-Induced Nucleation of Protein2013

    • Author(s)
      S. Nakayama, H. Yoshikawa, R. Murai, M. Kurata, M. Maruyama, S. Sugiyama, Y. Aoki, Y. Takahashi, M. Yoshimura, S. Nakabayashi, H. Adachi, H. Matsumura, T. Inoue, K. Takano, S. Murakami, Y. Mori
    • Journal Title

      Cryst. Growth Des.

      Volume: 13 Pages: 1491-1496

    • Peer Reviewed
  • [Journal Article] 固相ハイドロゲル中でのタンパク質結晶化2012

    • Author(s)
      杉山成、松村浩由、丸山美帆、佐崎元、廣瀬未果、安達宏昭、高野和文、村上聡、井上豪、森勇介
    • Journal Title

      日本結晶学会誌

      Volume: 54巻 Pages: 300-303

    • Peer Reviewed
  • [Journal Article] Growth of protein crystals in hydrogels prevents osmotic shocks2012

    • Author(s)
      S. Sugiyama, M. Maruyama, G. Sazaki, M. Hirose, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, and H. Matsumura
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 134 (13) Pages: 5786-5789

  • [Journal Article] Three-dimensional, non- invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography2012

    • Author(s)
      N. Nishizawa, S. Ishida, M. Hirose, S.Sugiyama, T. Inoue, Y. Mori, K. Itoh, and H. Matsumura
    • Journal Title

      Biomedical Optics Express

      Volume: 3 Pages: 735-740

  • [Presentation] 創薬スクリーニングを容易にするタンパク質結晶の機械的強化技術2013

    • Author(s)
      杉山成、松村浩由、丸山美帆子、佐崎元、廣瀬未果、安達宏昭、高野和文、村上聡、井上豪、森勇介
    • Organizer
      第24回高分子ゲル研究討論会
    • Place of Presentation
      東京大学 山上会館
    • Year and Date
      2013-01-16
  • [Presentation] 蛋白質の固相ゲル結晶育成技術の開発とその機能解析2012

    • Author(s)
      杉山成、安達宏昭、高野和文、村上聡、井上豪、森勇介、松村浩由
    • Organizer
      第42回結晶成長国内会議
    • Place of Presentation
      九州大学筑紫キャンパス
    • Year and Date
      2012-11-09
  • [Presentation] 超高分解能OCTによるタンパク質結晶の3次元・高機能イメージング2012

    • Author(s)
      石田周太郎、西澤典彦、廣瀬未果、杉山成、井上豪、森勇介、伊東一良、松村浩由
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      東京 タワーホール船堀
    • Year and Date
      2012-10-23
  • [Presentation] Hydrogel Protects Protein Crystals From Osmotic Shock2012

    • Author(s)
      S. Sugiyama, M. Maruyama, G. Sazaki, M. Hirose, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, and H. Matsumura
    • Organizer
      14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14)
    • Place of Presentation
      Huntsville, Arabama USA
    • Year and Date
      2012-09-27
  • [Presentation] Effect of solution stirring on protein crystal growth on high concentrated hydrogel2012

    • Author(s)
      Y. Aoki, M. Maruyama, Y. Takahashi, M. Yo sh imu r a, H. Yo sh ik awa, S. Sug iyama, H. Adachi, K. Takano, S. Murakami, H. Matsumura, T. Inoue and Y. Mori
    • Organizer
      14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14)
    • Place of Presentation
      Huntsville, Arabama USA
    • Year and Date
      2012-09-27
  • [Presentation] 超高分解能OCTによるタンパク質結晶の3次元・非侵襲断層イメージング2012

    • Author(s)
      石田周太郎、西澤典彦、廣瀬未果、杉山成、井上豪、森勇介、伊東一良、松村浩由
    • Organizer
      第73回応用物理学会学術講演会
    • Place of Presentation
      愛媛 松山大学文京キャンパス
    • Year and Date
      2012-09-12
  • [Presentation] Growth of Protein Crystals in Semi-Solid Hydrogel2012

    • Author(s)
      S. Sugiyama
    • Organizer
      Institute for Protein Research Seminar
    • Place of Presentation
      Osaka University
    • Year and Date
      2012-06-18
  • [Presentation] Towards an understanding of lipid-protein interactions, new approaches for producing high-quality protein crystals2011

    • Author(s)
      S. Sugiyama
    • Organizer
      International ERATO Symposium on Lipid Structures in and around Proteins
    • Place of Presentation
      Hotel Hankyu Expo Park
    • Year and Date
      2011-11-14

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Published: 2014-08-29  

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