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2012 Fiscal Year Final Research Report

Live-cell Imaging Analysis of Mouse Embryonic Stem Cell Derivation Processes

Research Project

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Project/Area Number 23870017
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionOsaka University

Principal Investigator

UEDA Jun  大阪大学, 微生物病研究所, 特任助教 (80450394)

Project Period (FY) 2011 – 2012
KeywordsES 細胞 / ライブセルイメージング / エピジェネティクス
Research Abstract

This project aims to identify the nature and origin of ES cells. More specifically, we wanted to know how ES cells are derived from inner cell mass cells (the events that take place during ES cell derivation), and what kind of cells they originated from. To address these questions, we decided to generate multiple reporter mice and carried out live-cell imaging analysis. One of the reporter mice which we generated can visualize DNA methylation status in living condition. This reporter mouse was termed MethylRO(methylation probe in ROSA26locus) and deposited to RIKEN BRC. Using MethylRO, we discovered that DNA methylation pattern undergoes dynamic changes during early embryonic development, whereas DNA methylation pattern was fairly static in ES cells. We are now preparing to carry out live-cell imaging analysis of ES cell derivation process using this MethylROmouse embryo (Ueda et al.In preparation).

  • Research Products

    (18 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (11 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] A Genetic and Developmental Pathway from STAT3 to the OCT4-NANOG Circuit is Essential for Maintenance of ICM Lineages in vivo.2013

    • Author(s)
      Do, D.V., Ueda, J., Messerschmidt, D.M., Lorthongpanich, C., Zhou, Y., Feng, B., Guo, G., Lin, P.J., Hossain, Z., Zhang, W., Moh, A., Wu, Q., Robson, P., Ng, H.H., Poellinger, L., Knowles, B.B., Solter, D. and Fu, X.Y.
    • Journal Title

      Genes & Dev

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Long-term Live-cell Imaging of Mammalian Preimplantation Development and Derivation Process of Pluripotent Stem Cells from the Embryos.2013

    • Author(s)
      Yamagata, K. and Ueda, J.
    • Journal Title

      Dev. Growth Differ

      Volume: 55 Pages: 378-389

    • DOI

      doi:10.1111/dgd.12048

    • Peer Reviewed
  • [Journal Article] PRDM14 Ensures Naive Pluripotency through Dual Regulation of Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells.2013

    • Author(s)
      Yamaji, M., Ueda, J., Hayashi, K., Ohta, H., Yabuta, Y., Kurimoto, K., Nakato, R., Yamada, Y., Shirahige, K., and Saitou, M.
    • Journal Title

      Cell Stem Cell

      Volume: 12 Pages: 368-382

    • DOI

      doi:10.1016/j.stem.2012.12.012

    • Peer Reviewed
  • [Journal Article] Dual Inhibition of Src and GSK3 Maintains Mouse Embryonic Stem Cells, Whose Differentiation Is Mechanically Regulated by Src Signaling.2012

    • Author(s)
      Shimizu, T., Ueda, J., Ho, J.C., Iwasaki, K., Poellinger, L., Harada, I., and Sawada, Y.
    • Journal Title

      Stem Cells

      Volume: 30 Pages: 1394-1404

    • DOI

      doi:10.1002/stem.1119

    • Peer Reviewed
  • [Presentation] Hypoxia-inducible Epigentic Regulators Jmjd1a and G9a Antagonistically Control Angiogenesis and Immature Tumorigenic Cell Populations2012

    • Author(s)
      Ueda, J., Lee, K.L., Ho, J.C., Kitajima, S., Yang, H., Sun, W., Fukuhara, N., Zaiden, N., Chan, S.L., Tachibana, M., Shinkai, Y., Kato, H., and Poellinger, L.
    • Organizer
      第10回がんとハイポキシア研究会
    • Place of Presentation
      横浜
    • Year and Date
      20121206-07
  • [Presentation] Visualization of DNA Methylation Status in Living Mice2012

    • Author(s)
      Ueda, J., Yamagata, K., Ikawa, M., and Okabe, M.
    • Organizer
      新学術領域研究「哺乳類初期発生の細胞コミュニティー」第5回班会議
    • Place of Presentation
      逗子
    • Year and Date
      20121119-21
  • [Presentation] Hypoxia-inducible Epigentic Regulators Jmjd1a and G9a Antagonistically Control Angiogenesis and Immature Tumorigenic Cell Populations.2012

    • Author(s)
      Ho, J.C., Lee, K.L., Ueda, J., Kitajima, S., Yang, H., Sun, W., Fukuhara, N., Zaiden, N., Chan, S.L., Tachibana, M., Shinkai, Y., Kato, H., and Poellinger, L.
    • Organizer
      Frontiers in Cancer Science 2012
    • Place of Presentation
      NUS, Singapore
    • Year and Date
      20121105-08
  • [Presentation] PRDM14 Controls Sensitivity to Extrinsic Signals and Ensures a Naive Epigenome in Mouse Embryonic Stem Cells.2012

    • Author(s)
      Yamaji, M., Ueda, J., Hayashi, K., Ohta, H., Yabuta, Y., Kurimoto, K., Nakato, R., Shirahige, K., and Saitou, M.
    • Organizer
      Cold Spring Harbor Meeting "Germ Cells"
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Year and Date
      20121002-06
  • [Presentation] A genetically encoded probe for monitoring the endogenous histone H3 Lys9 acetylation in living cells2012

    • Author(s)
      Sato, Y., Ueda, J., Horikoshi, N., Kujirai, T., Stasevich, T.J., Yamagata, K., Kurumizaka, H., Nozaki, N., and Kimura, H.
    • Organizer
      Cold Spring Harbor Meeting "Epigenetics & Chromatin"
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Year and Date
      20120911-15
  • [Presentation] ES 細胞のできるまで2012

    • Author(s)
      山縣一夫,上田潤,水谷英二,斎藤通紀,若山照彦
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      筑波
    • Year and Date
      20120905-08
  • [Presentation] Visualization of DNA Methylation Status in Living Mice2012

    • Author(s)
      Ueda, J., Yamagata, K., Ikawa, M., and Okabe, M.
    • Organizer
      生殖サイクル若手勉強会
    • Place of Presentation
      仙台
    • Year and Date
      20120725-27
  • [Presentation] The STAT3-OCT4 Axis is Essential for Development of Inner Cell Mass and Embryonic Stem Cells2012

    • Author(s)
      Do, D.V., Ueda, J., Lorthongpanich, C., Zhang, W., Moh, A., Zhou, Y., Feng, B., Guo, G., Lin, P.J., Hossain, Z., Messerschmidt, D.M., Wu, Q., Robson, P., Ng, H.H., Poellinger, L., Knowles, B.B., Solter, D. and Fu, X.Y.
    • Organizer
      International Society for Stem Cell Research, 10th Annual Meeting
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20120613-16
  • [Presentation] ヒストン修飾抗体 scFv を用いた H3K9 アセチル化レベルの生細胞イメージング2012

    • Author(s)
      佐藤優子,上田潤,山縣一夫,堀越直樹,胡桃坂仁志,野崎直仁,木村宏
    • Organizer
      第6回エピジェネティクス研究会年会
    • Place of Presentation
      東京
    • Year and Date
      20120514-15
  • [Presentation] Role of the Hypoxia-Inducible Histone H3K9 Demethylase Jmjd1a and the H3K9 Methyltransferase G9a in Stem Cell Self-Renewal and Tumorigenesis2012

    • Author(s)
      Ueda, J., Lee, K.L., Ho, J.C., Yang, H., Fukuhara, N., Chan, S.L., Tachibana, M., Shinkai, Y., Kato, H., and Poellinger, L.
    • Organizer
      Keystone Symposia "Advances in Hypoxic Signaling"
    • Place of Presentation
      Banff, Alberta, Canada
    • Year and Date
      20120212-17
  • [Presentation] Survival and death of epiblast cells in ES derivation process revealed by long-term live-cell imaging of Oct4 reporter system.2012

    • Author(s)
      Ueda, J., Yamagata, K., Mizutani, E., Saitou, M., and Wakayama, T.
    • Organizer
      定量生物学の会,第四回年会
    • Place of Presentation
      名古屋
    • Year and Date
      20120107-09
  • [Book] 細胞2013

    • Author(s)
      上田潤,三村維真理
  • [Remarks]

    • URL

      http://researchmap.jp/wiz/

  • [Patent(Industrial Property Rights)] Method to preserve the pluripotency of embryonic stem cells.2011

    • Inventor(s)
      Sawada, Y., Shimizu, T., and Ueda, J.
    • Industrial Property Rights Holder
      Sawada, Y., Shimizu, T., and Ueda, J.
    • Industrial Property Number
      United States Patent US Provisional Application No.: 61/495,232
    • Filing Date
      2011-06-09
    • Overseas

URL: 

Published: 2014-08-29  

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