2016 Fiscal Year Final Research Report
Molecular-scale functional visualization of bio- and nano-materials by AFM functional probes
| Project/Area Number |
24221008
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小林 圭 京都大学, 白眉センター, 特定准教授 (40335211)
平田 芳樹 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (10357858)
野田 啓 京都大学, 工学(系)研究科(研究院), 助教 (30372569)
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| Co-Investigator(Renkei-kenkyūsha) |
HIZUME Kohji 国立遺伝学研究所, 細胞遺伝研究系, 助教 (10378846)
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| Project Period (FY) |
2012-05-31 – 2017-03-31
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| Keywords | ナノ計測 / 原子間力顕微鏡 / 水和構造計測 / 3次元フォースマップ |
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| Outline of Final Research Achievements |
Based on both high-resolution frequency modulation atomic force microscopy (AFM) and dual probe AFM techniques, working in liquids, we have successfully realized a novel method for the visualization of molecular-scale bio-functions in addition to structural imaging of biomolecules. The method allows us to directly investigate various biomolecules in a wide variety of aspects such as direct visualization of protein domains, molecular-scale identification of specific binding sites and quantitative force analysis of binding interactions. Furthermore, we succeeded in the visualization of hydration structures and local charge densities of biomolecules, which are directly related to biochemical activities.
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| Free Research Field |
表面物理学, ナノ計測, 分子エレクトロニクス
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