Elucidation of mechanisms of biomaterial conversion mediated by amino group-modifying carrier protein and application

2017 Fiscal Year Final Research Report

• Project/Area Number: 24228001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: NISHIYAMA MAKOTO 東京大学, 生物生産工学研究センター, 教授 (00208240)
• Co-Investigator(Kenkyū-buntansha): 富田 武郎 東京大学, 生物生産工学研究センター, 助教 (50447364)
• Project Period (FY): 2012-05-31 – 2017-03-31
• Keywords: キャリアタンパク質 / 生合成 / リジン生合成 / アルギニン生合成 / 二次代謝生合成 / 結晶構造解析 / 天然物化学

Outline of Final Research Achievements

In this study, we found that lysine biosynthesis via a carrier protein (AmCP) that binds to the amino group we have previously found is widely distributed in the thermophilic archaea with evolutionarily old origins, and that it is also utilized as arginine biosynthesis system, suggesting that the system is the key to clarifying the biosynthesis evolution. We also succeeded in elucidating the crystal structures of complexes of various biosynthetic enzymes and AmCP, revealing that they recognize each other by electrostatic interaction for efficient reaction. Furthermore, we discovered that biosynthesis diverted from this system is also used for secondary metabolism of actinomycetes. All these results also clarified that the system plays an important role as a biosynthesis system.

Free Research Field

応用微生物学