Dynamic roles of postsynaptic density in glutamate receptor trafficking

2014 Fiscal Year Final Research Report

• Project/Area Number: 24300140
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Hokkaido University
• Principal Investigator: KAMIYA Haruyuki 北海道大学, 医学(系)研究科(研究院), 教授 (10194979)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脳・神経 / 神経科学 / 生理学 / 薬理学 / シグナル伝達

Outline of Final Research Achievements

In this study, we examined the dynamics of endogenous AMPA-type glutamate receptors at the intact synapses in acute hippocampal slices using photochemical inactivation by using photoreactive AMPA receptor antagonist ANQX. Postsynaptic AMPA receptors are less mobile at the resting condition, and the synaptic delivery from intracellular reserve pools were accererated during induction of long-term potentiation. We also adopted photochemical inactivation in hippocampal slices obtained from knock out mice of PSD95, one of the major proteins of postsynaptic density. In PSD95 kockout mice, synaptic delivery were accererated during the resting condition than wild type mice. These results suggested that PSD95 serves as a receptor slot to limit the mobility of AMPA receptors during the resting condition, and may upregulated after the induction of long-term potentiation to increase the synaptic efficacy.

Free Research Field

神経生理学