Cellular response to DNA single-strand break and its repair

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Tohoku University
• Principal Investigator: YASUI Akira 東北大学, 加齢医学研究所, 教授 (60191110)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: single-strand break / DNA repair / PARP1 / Poly(ADP-ribosyl)ation / MMS / Homologous recombination

Outline of Final Research Achievements

DNA single-strand break is thought to be the most frequent type of DNA damage, but its significance in biological function and its repair within human cell remain to be determined. Here we identified genes involved in the regulation of SSB repair in human cell. ONe gene encodes a protein, which interacts directly with PARP1 within human cell. When its expression was suppressed with siRNA, poly(ADP-ribiosyl)ation was decreased after treatment of cells with MMS and cellular resistance to MMS decreased. Its recombinant protein has an endonuclease against AP site as well as exonuclease to both 5' and 3' directions. These data suggest that while PARP1 is able to bind SSB, for its effective activation PARP1 requires gap produced by this protein. Further analysis is required to elucidate the importance of this protein in cellular survival and oncogenesis.

Free Research Field

DNA修復と分子生物学