2015 Fiscal Year Final Research Report
Analysis of the relationship between DNA double strand break repair pathway and mutagenesis in response to ionizing radiation by using novel assay systems
| Project/Area Number |
24310038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | DNA修復 / 体細胞突然変異 / 放射線感受性 |
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| Outline of Final Research Achievements |
DNA double strand break (DSB), which is often induced by ionizing radiation, is one of the most serious DNA damage. To clarify the relationship between DSB repair pathway and mutation induction, we developed a site-specific DSB induction system by using artificial nucleases. We analyzed DSB repair efficiency and mutation spectrum at the DSB site, and found that efficiency and accuracy of DSB repair could be separately regulated. We also found that mutation at FHA domain of NBS1 renders the cells to be sensitive to radiation without increase in mutation frequency. A part of our findings were reported as several papers in scientific journals.
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| Free Research Field |
放射線生物学
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