Development of real-time nanometer-resolved scanning electron optical microscope based on television imaging tube technologies

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310096
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: National Institute for Materials Science
• Principal Investigator: MIYAZAKI Hideki 独立行政法人物質・材料研究機構, 先端フォトニクス材料ユニット, グループリーダー (10262114)
• Research Collaborator: KASAYA Takeshi
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 電子顕微鏡 / 光物性 / 可視化

Outline of Final Research Achievements

We revived classical television imaging tube technologies by use of modern nanotechnologies and developed a scanning electron optical microscope that enables us super-resolved optical observation of unstained specimen in the near-field of the imaging surface with a resolution of 62 nm. We considered the construction of an iconoscope, in which the accumulated charges due to the photoelectron emission are read out, and a flying spot scanner, in which a scanned fluorescent spot illuminates the specimen. Although the former is still under development, the latter was successfully completed in the form of a unit attachable to the specimen chamber of commercialized scanning electron microscopes. Luminescent environmental cells that enables the super-resolved observation of living biological cells were developed. The image formation principle of unstained phase objects was determined to be the Purcell effect. Finally unstained living human lung epithelial cells in liquid was observed.

Free Research Field

複合新領域