2015 Fiscal Year Final Research Report
Novel high-sensitivity detection method of virus using a hairpin primer PCR
Project/Area Number |
24310165
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Chemical biology
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Research Institution | National Defense Medical College (2014-2015) Osaka University (2012-2013) |
Principal Investigator |
Fumie Takei 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科進学課程, 准教授 (30252711)
|
Co-Investigator(Renkei-kenkyūsha) |
NAKAKTNI Kazuhiko 大阪大学, 産業科学研究所, 教授 (70237303)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Keywords | PCR / 蛍光分子 / 遺伝子検出 / ウイルス検出 / グアニン / シトシンバルジ構造 / RT-PCR |
Outline of Final Research Achievements |
We have previously described a hairpin primer PCR (HP-PCR) method using a primer having a hairpin tag at the 5′ end and a fluorescent dye (2,7-diamino-1,8-naphthyridine derivative [DANP]) bound to the cytosine bulge (C-bulge) embedded in the hairpin-tag. It was turn-off type of system. In this research, we tried to develop the high-sensitivity detection method using a HP-PCR and it applied to detect some kinds of virus. In this research, we developed the new type of PCR monitoring system using DANP bound to the primers as a covalent and in which the fluorescent signal increases as PCR progresses. This was a turn-on of PCR using fluorescently and modified DNA.
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Free Research Field |
ゲノム化学
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