Basic measurement of biomembranes: characterization as two-dimensional reaction field

2014 Fiscal Year Final Research Report

• Project/Area Number: 24350012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Gakushuin University
• Principal Investigator: IWATA Koichi 学習院大学, 理学部, 教授 (90232678)
• Co-Investigator(Kenkyū-buntansha): TAKAYA Tomohisa 学習院大学, 理学部, 助教 (70466796) ; NAKAMURA Hiroyuki 東京工業大学, 資源化学研究所, 教授 (30274434)
• Research Collaborator: NOJIMA Yuki ; MOHRI Goh ; KITAMURA Sho
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 生体膜 / リポソーム / 時間分解分光 / ラマン / 化学反応

Outline of Final Research Achievements

We examine lipid bilayer membranes as a field of chemical reactions, by estimating their thermal diffusivity, viscosity, and polarity, with time-resolved spectroscopies. Picosecond time-resolved Raman spectroscopy of trans-stilbene solubilized in lipid bilayer membranes reveals that the rate of energy transfer changes when the phospholipid that forms the lipid bilayer or the temperature changes. Picosecond time-resolved fluorescence spectroscopy indicates that there are two environments with viscosity different by 30 to 290 times in the lipid bilayer membranes. We have developed new phospholipids by introducing fluorophores into phosphatidylcholines for examining the properties of lipid bilayer membranes at a fixed depth.

Free Research Field

分光物理化学