2014 Fiscal Year Final Research Report
Compilation of heme oxygenase catalysis
| Project/Area Number |
24350081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOSHITAKA Matsui 東北大学, 多元物質科学研究所, 准教授 (90323120)
MASAKI Unno 茨城大学, 理工学研究科, 教授 (10359549)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ヘム / 酸素活性化 / 結晶構造解析 / 反応機構 / 共鳴ラマン散乱 / EPR / 分子動力学 |
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| Outline of Final Research Achievements |
Heme oxygenase (HO) converts heme into biliverdin, CO, and iron by three step monooxygenation reactions. Crystal structures of the heme-free, iron-biliverdin and biliverdin complexes have been solved, and the HO protein structural changes associated with the heme-binding and biliverdin-release have been determined by the molecular dynamic simulations. In addition, the final reaction products of a new type heme degradation enzyme, MhuD of M. tuberculosis and its homologue IsdG of S. aureus, have been established. We have found that that these enzyme degrade heme without CO release, demonstrating a new way to degrade heme.
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| Free Research Field |
生物無機化学
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