Development of A General Single-Molecular Detection System Utilizing Nanomechanical DNA Origami Devices

2015 Fiscal Year Final Research Report

• Project/Area Number: 24350088
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Kansai University
• Principal Investigator: Kuzuya Akinori 関西大学, 化学生命工学部, 准教授 (00456154)
• Co-Investigator(Kenkyū-buntansha): OHYA Yuichi 関西大学, 化学生命工学部, 教授 (10213886)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: DNAオリガミ / 単分子検出 / 生体分子 / DNAナノテクノロジー / 核酸化学 / 分子ロボティクス

Outline of Final Research Achievements

A single-molecular detection system utilizing our original nanomechanical DNA origami devices (DNA pliers) composed of two 170-nm long stick-like components connected to each other at a fulcrum has been applied to more practical use by improving the molecular design and nanomechanical properties. DNA pliers can now detect protons, theoretically smallest substance, as well as special binding event of bio-related molecules such as PNA strand invasion. Introduction of a tubular design enabled handy detection of structure change of DNA pliers with popularly available gel electrophoresis.

Free Research Field

生体超分子化学、分子ロボティクス、核酸化学