In vitro assay system for evaluating curative effect of myoblast sheet transplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24360341
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: KINO-OKA Masahiro 大阪大学, 工学(系)研究科(研究院), 教授 (40234314)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞シート / 筋芽細胞 / 血管新生 / 細胞アッセイ / 流動性

Outline of Final Research Achievements

Autologous transplantation of myoblast sheet has attracted attention as a new technique for curing myocardial infarction. Myoblast sheet has the ability to secret cytokines which improve heart function via the facilitation of angiogenesis on affected part. To understand in vivo phenomena and evaluate curative effect after transplantation such as cytokine secretion from transplant and its angiogenesis capability, a multi-layered cell sheet of human skeletal muscle myoblasts (HSMMs) was constructed by originally equipped gelatin stamp system. In the HSMM sheet, active cellular migration occurred in the horizontal and vertical directions anywhere, revealing the sheet fluidity. Dynamic behavior including collective migration and spatial habitation of heterogeneous cells, such as human skeletal muscle fibroblasts (HSMFs) and human umbilical vein endothelial cells (HUVECs), in the fluidic scaffold was clarified.

Free Research Field

工学