2015 Fiscal Year Final Research Report
Diversity and molecular evolution of eukaryotic electron transfer mechanisms
Project/Area Number |
24380041
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | University of Tsukuba |
Principal Investigator |
TAKAYA Naoki 筑波大学, 生命環境系, 教授 (50282322)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | NAD / NADH / Nudix hydrolase |
Outline of Final Research Achievements |
The filamentous fungus Aspergillus nidulans controls intracellular redox homeostasis by shuttling electron equivalents through cell membrane. Cellular NAD+/NADH level maintains the homeostasis and various fungal cell functions. This study employs A. nidulans as a model fungus, and revealed novel controlling mechanism of cellular redox state. I revealed that the Nudix hydrolase AnNUDT1 (NdxA) is a key controlling protein of cellular redox state. NdxA hydrolyzes NAD+/NADH, which affects rates of cellular thiamine biosynthesis and glycolysis, both of which use NAD+ as a substrate. NAD+ level also affects function of NAD+-dependent histone deacetylase isozyme (sirtuin A), and histone acetylation level, and hence fungal secondary metabolisms. These results shed light novel NdxA and NAD+/NADH function in the cellular processes, and contribute improved use of filamentous fungi in industry.
|
Free Research Field |
応用微生物学
|