Gibberellin-like diterpenoids involved in differentiation of moss protonemata

2015 Fiscal Year Final Research Report

• Project/Area Number: 24380060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Nakajima Masatoshi 東京大学, 農学生命科学研究科, 准教授 (50237278)
• Co-Investigator(Kenkyū-buntansha): KAWAIDE Hiroshi 東京農工大学, 大学院農学研究院, 准教授 (20291916)
• Co-Investigator(Renkei-kenkyūsha): HAYASHI Ken-ichiro 岡山理科大学, 理学部, 教授 (30289136)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 植物 / ホルモン / 生理活性物質 / コケ / 分化制御

Outline of Final Research Achievements

A kind of moss (Physcomitrella patens) has a partial biosynthetic pathway of gibberellin, that is, an ent-kaurene synthase PpCPS/KS catalyzes a cyclization reaction from geranylgeranyl diphosphate to ent-kaurene, and then an ent-kaurene oxidase PpKO does from the ent-kaurene to ent-kaurenoic acid. However, the following pathway toward GAs is not identified in the moss. In our previous studies, we showed that the functional disruption of PpCPS/KS leads to the suppression of protonemal differentiation under the continuous red-light, and the phenotype was complemented by the application of either ent-kaurene or ent-kaurenoic acid, but not by that of gibberellin A(4). By using a bioassay system with the Ppcps/ks mutants, we identified the biologically-active metabolite(s) derived from ent-kaurenoic acid. We expect that any of the metabolite(s) should regulate the protonemal differentiation per se. The structural analyses of the metabolite(s) are now proceeding.

Free Research Field

活性生理化学・天然物化学