Biosynthetic enzymes of antifouling halogenated compounds

2015 Fiscal Year Final Research Report

• Project/Area Number: 24380112
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Hokkaido University
• Principal Investigator: Okino Tatsufumi 北海道大学, 地球環境科学研究科(研究院), 准教授 (30280910)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Fuyuhiko 北海道大学, 大学院地球環境科学研究院, 教授 (10219446) ; UMEZAWA Taiki 北海道大学, 大学院地球環境科学研究院, 准教授 (20503618)
• Co-Investigator(Renkei-kenkyūsha): NOGATA Yasuyuki 電力中央研究所, 環境科学研究所, 主任研究員 (10371535) ; WASHIO Kenji 北海道大学, 大学院地球環境科学研究院, 助教 (50241302)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 生合成 / 付着阻害 / フジツボ / 紅藻 / ブロモアレン / キプリス / ブロモペルオキシダーゼ / テルペノイド

Outline of Final Research Achievements

Vanadium dependent bromoperoxidase (VBPO) was cloned from the red alga Laurencia nipponica which produces a halogenated compound, laurencin. The enzyme catalyzed cyclization and bromination of a precursor of laurencin. Bromination activity of recombinant enzyme was investigated. A similar type of VBPO was cloned from L. okamurae which produces a halogenated sesquiterpenoid, laurinterol. The recombinant enzyme showed bromination activity. In addition, another type of VBPO was cloned from L. saitoi which produces a halogenated triterpenoid. Its properties were investigated by using its recombinant protein. The structure of bromoallene containing C15 compound, omaezallene was reported. Its totaly syntehsis was successfully achieved.

Free Research Field

海洋天然物化学