Role of glycans in chronic inflammation revealed by novel anti-carbohydrate antibodies

2015 Fiscal Year Final Research Report

• Project/Area Number: 24390018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Chiba University (2015); Hoshi University (2014); University of Shizuoka (2012-2013)
• Principal Investigator: KAWASHIMA Hiroto 千葉大学, 薬学研究科(研究院), 教授 (50260336)
• Co-Investigator(Kenkyū-buntansha): IMAI Yasuyuki 静岡県立大学, 薬学部, 教授 (80160034)
• Co-Investigator(Renkei-kenkyūsha): KOBAYASHI Motohiro 福井大学, 医学部, 教授 (00362137)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: リンパ球ホーミング / 抗糖鎖抗体 / シアリルルイスX / L-セレクチン / P-セレクチン / E-セレクチン / 糖鎖 / 炎症

Outline of Final Research Achievements

In this study, we succeeded in the generation of novel monoclonal antibodies reactive with a fucosylated glycan epitope sialyl Lewis X based on a newly developed method. In mouse contact hypersensitivity model, both F1 and F2 significantly inhibited ear swelling by blocking sialyl Lewis X-dependent leukocyte infiltration upon antigen challenge in sensitized animals. In silicosis model, these monoclonal antibodies inhibited lung fibrosis at the chronic stage by blocking sialyl Lewis X-dependent acute leukocyte infiltration after administration of silica. Taken together, these results indicate that functional blockage of sialyl Lewis X at particular stage of inflammation leads to efficient control over inflammatory disorders.

Free Research Field

免疫学、生化学