2014 Fiscal Year Final Research Report
Owing to the development of RNA medicine, discovery of practical RNA molecules and synthesis of nucleic acid PET probes
Project/Area Number |
24390025
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Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Drug development chemistry
|
Research Institution | Gifu University |
Principal Investigator |
KITADE Yukio 岐阜大学, 工学部, 教授 (20137061)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | RNA創薬 / オリゴヌクレオチド / クリック反応 / ラベル標識化 / 薬物動態 / PETプローブ / バイオイメージング / ドラッグデリバリー |
Outline of Final Research Achievements |
Ethynylbenzene-substituted glycol, as a versatile crick-reaction probe, was synthesized and their RNA post-labeling property was investigated. Stereoselective synthesis of 1-deoxy-1-ethynyl-β-D-ribofuranose was performed for RNA labeling. SiRNAs and miRNAs bearing urea/thioures-bridged aromatic compounds at their 3'-end were also prepared and these dsRNAs definitely showed nuclease resistance. Oligonucleotides with glucosamine at the 3'-position were synthesized reaction and their biological activity was investigated. Some of chemically modified siRNAs at the 3’-position showed avoidance of off-target effect. Chemically modified synthetic miRNA-205 inhibited the growth of melanoma cells in vitro and in vivo. A clinical trial of miR-205 against canine melanoma is now in progress; thus far, intratumoral injection of miR-205 into a recurrent tumor derived from naturally occurring canine melanoma has resulted in complete remission in 2 of 5 cases.
|
Free Research Field |
医歯薬学
|