2014 Fiscal Year Final Research Report
Genotype-dependent regulation of drug-metabolizing enzymes by microRNAs
| Project/Area Number |
24390039
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Research Collaborator |
NAKANO Masataka
IWAKAMI Chika
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | マイクロRNA / 遺伝子変異 / 個人差 / 転写後調節 / 薬物代謝酵素 / 薬物動態 |
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| Outline of Final Research Achievements |
Genetic polymorphisms of drug-metabolizing enzymes are relevant to interindividual differences in pharmacokinetics, drug response and toxicity. Effects of SNPs in the coding region and 5’-UTR on enzyme activities or expression levels have been well studied, whereas SNPs in the 3’-UTR have been overlooked. Some SNPs in the 3’-UTR of concerned genes are associated with the change in the expression level. In this study, we found that human CYP2E1, AKR1D1, and TYMS are regulated by microRNA in the genotype-dependent manner. This is the first proof that a SNP(s) in the 3’-UTR of drug-metabolizing enzymes affects binding of miRNA to modulate the expression in the liver.
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| Free Research Field |
薬物代謝
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