2014 Fiscal Year Final Research Report
Analysis of propagation mechanisms of hepatitis C virus and establishment of novel permissive cell lines
| Project/Area Number |
24390113
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | HCV / replication / permissive cell line |
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| Outline of Final Research Achievements |
Previous studies have shown that liver-specific host factors are required for efficient replication of HCV RNA and formation of infectious particles. In this study, we screened human cancer cell lines for expression of the liver-specific α-fetoprotein by using a cDNA array database, and identified novel permissive cell lines for a complete propagation of HCVcc without any artificial manipulation. In particular, gastric cancer-derived FU97 cells exhibited a much higher susceptibility to HCVcc/JFH-2 infection than was observed in Huh7 cells, suggesting that FU97 cells would be useful for further investigation of the HCV lifecycle, as well as the development of therapeutic agents for chronic hepatitis C.
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| Free Research Field |
ウイルス学
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