Development of PNA-based chromatography for rapid detection of respiratory viruses

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390146
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: NAKAYA TAKAAKI 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80271633)
• Co-Investigator(Kenkyū-buntansha): KAIHATSU Kunihiro 大阪大学, 産業科学研究所, 特任准教授 (70419464)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 迅速診断 / デバイス / インフルエンザウイルス / ペプチド核酸

Outline of Final Research Achievements

In this study, we developed a novel chromatography kit for the subtype-specific detection of influenza A viruses. Azobenzene-tethered hairpin-type peptide nucleic acid (Tail-clamp PNA-AZO) possessing a subtype-specific complementary sequence against human influenza A virus, H1-, H3-, and avian H7-subtype, was synthesized and attached to a chromatography strip. The sensitivity of the chromatography was approximately 1.0×104 ffu/kit for H1N1 and H3N2, and 5.0×105 ffu/kit for H7N7, respectively. The PNA-chromatography specifically detected each influenza A virus tested, although it slightly reacted with other subtypes.
Even though further improvement is necessary to use it clinically as a rapid diagnostic kit, this system could be applied for the sequence-specific detection of influenza viruses, including drug-resistant viruses as well as highly-pathogenic avian influenza viruses.

Free Research Field

ウイルス学