Study of biology and regulation of vitreous body

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390396
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kagoshima University
• Principal Investigator: SAKAMOTO Taiji 鹿児島大学, 医歯学総合研究科, 教授 (10235179)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Ikuro 鹿児島大学, 医歯学総合研究科, 教授 (20082282) ; HASHIGUCHI Teruto 鹿児島大学, 医歯学総合研究科, 教授 (70250917) ; TAKAO Sonshin 鹿児島大学, 学内共同利用施設等 (80171411) ; KOSAI Kenichirou 鹿児島大学, 医歯学総合研究科, 教授 (90258418)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 硝子体 / ヒアルロン酸 / 細胞破壊物 / 炎症 / 硝子体手術 / ヒアルロン酸

Outline of Final Research Achievements

Biology of vitreous body was investigated during the study period. First, molecular profile of vitreous body was evaluated by measuring such molecules as HMGB-1 and histone H3. The results showed that both were rich in the vitreous with retinal detachment. The co-incubation of HMGB-1 and histone H3 with retinal cells increased the production of inflammatory cytokines by these cells. Furthermore, the apoptosis was induced when added excessively. These indicated that the damage in retinal disease does not only cause functional impairment of the damaged retina, but also induces collateral damage to the surrounding retina. To prevent this complication, we found that hyaluronic acid was most effective, which lead to development of novel surgical method termed “soft shell vitrectomy”. In each project, the detailed cellular mechanisms such as involvement of toll-like receptors were also studied.

Free Research Field

眼科学