Comprehensive in situ analysis of dental biofilm formation and control

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390425
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: EBISU Shigeyuki 大阪大学, 歯学研究科(研究院), 理事・副学長 (50116000)
• Co-Investigator(Kenkyū-buntansha): NOIRI Yuichiro 大阪大学, 大学院歯学研究科, 准教授 (50218286) ; KINOMOTO Yoshifumi 大阪大学, 大学院歯学研究科, 招へい教員 (10252694) ; YAMAGUCHI Mikiyo 大阪大学, 歯学部付属病院, 医員 (30523089) ; ASAHI Youko 大阪大学, 歯学部付属病院, 助教 (50456943) ; TAKAHASHI Yusuke 大阪大学, 大学院歯学系研究科, 助教 (60397693)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細菌 / デンタルバイオフィルム / in situ / メタゲノム解析 / ハイドロキイアパタイト / 共焦点レーザー顕微鏡

Outline of Final Research Achievements

Human dental biofilm (DB), which is different from the biofilms in vitro, contains more than 700 species of bacteria, and is influenced by the oral environment and host factors. The purpose of this research was to form DB in human oral cavity, to establish an experimental model of DB that can be evaluated, and to examine the formation mechanism and control method. In 2012, experimental model of DB formation on the apatite disc was developed. In 2013, quantitative analysis and morphological observation of the DB bacteria was conducted. In 2014, it is elucidated that bacteria number was increased in biphasic action by day 4, and obligate anaerobic bacteria genus was increased in late by qualitative analysis using metagenomic analysis.

Free Research Field

歯科保存学