Asymmetric stem cell division in the homeostasis and the disease onset of periodontal tissue

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390466
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Osaka University
• Principal Investigator: YAMASHITA MOTOZO 大阪大学, 歯学部附属病院, 助教 (90524984)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Shinya 大阪大学, 歯学研究科(研究院), 教授 (70239490) ; YANAGITA Manabu 大阪大学, 歯学研究科(研究院), 助教 (80379081) ; TAKEDACHII Masahide 大阪大学, 歯学研究科(研究院), 助教 (60452447)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 歯周組織幹細胞 / ニッチェ / 非対称性細胞分裂 / ECM蛋白

Outline of Final Research Achievements

An asymmetric cell division is crucial for the pool of the tissue stem cells and homeostasis of the organs. To dissect role of the asymmetric stem-cell divisions in the periodontal tissue, we first established the induction model for the senescence in human periodontal ligament (HPDL) cells in the primary culture. HPDL cells showed the decreased ratio of the SSEA3/4,ES cell marker, positive subpopulations depends on the progression of the cell dividing. Trans OMICS approach involving the analysis of transcriptome and proteome identified the global change of profiles in extra cellular matrix (ECM) protein species for the stem-cells exhaustion in senescent HPDL cells. We suggest the proper ECM synthesis is indispensable for the cellular communications in the stem cells/ niches in the homeostasis of periodontal tissue.

Free Research Field

歯周病系治療学