2014 Fiscal Year Annual Research Report
チェルノブイリ小児甲状腺癌のパラフィン組織バンク設立と新規癌遺伝子解析
| Project/Area Number |
24406031
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| Research Institution | Nagasaki University |
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Principal Investigator |
ログノビッチ タチアナ 長崎大学, 原爆後障害医療研究所, 助教 (30423643)
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| Co-Investigator(Kenkyū-buntansha) |
サエンコ ウラジミール 長崎大学, 原爆後障害医療研究所, 准教授 (30343346)
柴田 義貞 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (40010954)
中島 正洋 長崎大学, 原爆後障害医療研究所, 教授 (50284683)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 小児甲状腺乳頭癌 / 次世代シーケンシング / 新規癌遺伝子 |
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| Outline of Annual Research Achievements |
During 26年 we accomplished collection of blood samples by dispatching specialists to the affected after Chernobyl fallout areas, the development of database, and performed DNA extraction from all collected blood samples.
We finished biostatistical analysis of obtained data of DNA-exome sequence (point mutations) and mRNA from whole genome sequencing data (expressed rearrangements and point mutations).
We discovered a novel chimeric gene, which was a result of balanced chromosomal translocation t(3;10) (q26.32;q11.21). The 5’ portion of the gene was presented by the first 9 exons of TBL1XR containing a LisH region, and the 3’portion was the tyrosine kinase domain of RET. The breakpoint in the RET gene on the mRNA level localized in the same place as in RET/PTC1 and RET/PTC3 in exon 12 of RET. We also found the identical TBL1XR1/RET rearrangement in 1 case among 34 (2.9%) of sporadic pediatric PTCs from Belarus. We also already confirmed that in vitro exposure of normal human thyroid cells (Nthy-ori 3-1) to 1 Gy of γ-radiation resulted in the formation of same rearrangement. The newly found TBL1XR1/RET rearrangement may likely be induced by genotoxic insult, one of which could be ionizing radiation.
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| Research Progress Status |
26年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
26年度が最終年度であるため、記入しない。
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| Causes of Carryover |
26年度が最終年度であるため、記入しない。
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| Expenditure Plan for Carryover Budget |
26年度が最終年度であるため、記入しない。
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[Journal Article] The common genetic variant rs944289 on chromosome 14q13.3 associates with risk of both malignant and benign thyroid tumors in the Japanese population.2015
Author(s)
Rogounovitch TI, Bychkov A, Takahashi M, Mitsutake N, Nakashima M, Nikitski AV, Hayashi T, Hirokawa M, Ishigaki K, Shigematsu K, Bogdanova T, Matsuse M, Nishihara E, Minami S, Yamanouchi K, Ito M, Kawaguchi T, Kondo H, Takamura N, Ito Y, Miyauchi A, Matsuda F, Yamashita S, Saenko VA.
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Journal Title
Thyroid
Volume: 25
Pages: 333-340
DOI
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Morphological difference in adult thyroid papillary carcinoma between Japan and Ukraine.2014
Author(s)
Ito M, Bogdanova T, Zurnadzhy L, Saenko V, Rogounovitch T, Mitsutake N, Kondo H, Maeda S, Nakashima M, Tronko M, Yamashita S.
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Journal Title
Endocr J.
Volume: 61
Pages: 1221-1228
DOI
Peer Reviewed
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[Presentation] Association of polymorphism in the FOXE1 gene locus with papillary thyroid carcinoma in Japanese patients2014
Author(s)
V.Saenko, T.Rogounovitch, A.Bychkov, N.Mitsutake, M.Matsuse, E.Nishihara, M.Hirokawa, A.Nikitsky, S. Minami, K.Yamanouchi, K.Yoshiura, A.Miyauchi, S.Yamashita
Organizer
第87回日本内分泌学会学術総会
Place of Presentation
福岡国際会議場（福岡県 福岡市）
Year and Date
2014-04-24 – 2014-04-26
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[Presentation] Rare T allele of rs944289 associates not only with risk of malignant but also of benign thyroid tumors2014
Author(s)
Rogounovitch TI, Saenko VA, Bychkov A, Nikitski AV, Takahashi M, Nakashima M, Hayashi T, Hirokawa M, Miyauchi A, Ishigaki K, Shigematsu K, Matsuse M, Mitsutake N, Nishihara E, Minami S, Yamanouchi K , Ito M, Yoshiura K , Matsuda F, Yamashita S
Organizer
第87回日本内分泌学会学術総会
Place of Presentation
福岡国際会議場（福岡県福岡市）
Year and Date
2014-04-24 – 2014-04-26
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