2015 Fiscal Year Final Research Report
Biogeochemical characteristics of oligosaccharides in seawater as model compounds of low-molecular weight dissolved organic matter
Project/Area Number |
24510013
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Environmental dynamic analysis
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Research Institution | Shizuoka University |
Principal Investigator |
Sohrin Rumi (福田留美) 静岡大学, 理学部, 准教授 (00343195)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Keywords | オリゴ糖 / 海洋生態系 / 生元素循環 |
Outline of Final Research Achievements |
For better understanding of the dynamics of low-molecular weight dissolved organic matter in marine environments, we developed a novel technique to determine oligosaccharides concentrations in seawater. Seawater samples were desalted by solid phase extraction using with a graphite carbon column and evaporated to concentrate oligosaccharides, then analyzed by high performance anion exchange chromatography-pulsed amperometric detection. We determined the concentrations of sucrose, maltose, trehalose and lactulose in the epipelagic layer of the Suruga Bay, and detected other six unknown-oligosaccharides. MPN survey indicated high abundance of prokaryotes in the Suruga Bay that were grown on sucrose, maltose and trehalose. Our findings demonstrated, for the first time, bottom-up controls of prokaryotes by dissolved organic matter on a molecular level in seawater.
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Free Research Field |
生物海洋化学
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