2014 Fiscal Year Final Research Report
Molecular analysis of the pathway of non-homologous end-joining involved in the radioadaptive response
| Project/Area Number |
24510064
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 放射線適応応答 / 非相同末端結合 / DNAリガーゼ |
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| Outline of Final Research Achievements |
The radioadaptive response is the induction of cellular resistance to moderate or high doses of radiation by previous exposure to low doses. It has been suggested that the frequency of mis-rejoining of double-strand breaks (DSBs) is reduced in the radioadaptive response. There are two non-homologous end-joining (NHEJ) pathways to directly rejoin two ends of the breakpoint of DNA; classical NHEJ (C-NHEJ) and alternative NHEJ (alt-NHEJ). It is also known that DNA ligase IV is involved in C-NHEJ, and DNA ligase III in alt-NHEJ. In order to clarify which pathway is involved in the radioadaptive response, we investigated the radioadaptive response in the cells with reduced DNA ligase activity. We found that the radioadaptive response was suppressed in the cells with reduced amount of DNA ligase IV. On the contrary, the radioadaptive response was not suppressed in the cells with reduced amount of DNA ligase III. These results suggest that C-NHEJ is involved in the radioadaptive response.
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| Free Research Field |
放射線生物学
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