2014 Fiscal Year Final Research Report
Approaches for elucidation of structure-function relationships of modular chitinases using a novel modeling method
| Project/Area Number |
24510302
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KEZUKA Yuichiro 岩手医科大学, 薬学部, 助教 (50397163)
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| Co-Investigator(Renkei-kenkyūsha) |
SEKI Yasutaka 岩手医科大学, 薬学部, 准教授 (30377220)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | キチナーゼ / 抗真菌活性 / モデリング |
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| Outline of Final Research Achievements |
A bacterial chitinase (Chitinase C) is composed of two functional domains connected by a flexible linker. We prepared two kinds of mutant enzyme whose linker is elongated or replaced, and measured their antifungal and hydrolyzing activities. Although mutations in the linker were expected to vary the relative orientation of two domains, they affected the activities by up to only 25%. Furthermore, we developed a novel method to model protein structures with flexible interdomain linkers and select model structures as conformational ensembles on the basis of solution X-ray scattering data. The chitinase structures selected by this method can explain an action in chitin hydrolyzing we proposed previously.
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| Free Research Field |
蛋白質結晶学
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