Relationship between precursor folding and molecular evolution of a peptide hormone

2015 Fiscal Year Final Research Report

• Project/Area Number: 24510310
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Kinki University
• Principal Investigator: HIDAKA Yuji 近畿大学, 理工学部, 教授 (70212165)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI Hiroshi 関西学院大学, 理工学部, 教授 (10252719) ; SHIMAMOTO Shigeru 近畿大学, 理工学部, 講師 (00610487)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: シャペロン / ジスルフィド結合 / フォールディング / ペプチドホルモン / 前駆体 / ウログアニリン

Outline of Final Research Achievements

To investigate how peptide hormones maintain/develop their biologically active conformation during molecular evolution, the folding of precursor proteins including prouroguanylin were estimated in vitro. Our results indicated that i)the stability of the biologically active conformation of the mature region is strongly related to the correct folding of their precursor proteins, ii)precursor protein possesses a region to accelerate molecular evolution, iii)precursor protein possesses a region to inhibit molecular evolution, iv)correct folding of prevursor protein requires mis-folded molecular species to establish the correct conformation of prouroguanylin

Free Research Field

蛋白質・ペプチド科学