2014 Fiscal Year Final Research Report
High throughput gene-targeting with NHEJ deficient cells in plant
| Project/Area Number |
24570004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 遺伝子ターゲッティング / 植物 / バイオテクノロジー / 育種学 |
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| Outline of Final Research Achievements |
In the research, we will show the sure availability of a new system, which we had published in 2010, of gene targeting with NHEJ deficient cells in plant for analysis of gene function and plant bleeding.
We had tried that a target gene tagged with GFP gene is expressed from LIG4 deficient A. thaliana, and gene targeting is performed in either KU80 deficient or KU70 knocked-down plants. Because of problems, may be, from either condition of calli culture or constructs for gene manipulation, neither their transformed plants nor results have not yet been obtained. But, we observed shoot formation from our cultured calli. These experiments will do continuously after addition of both some checks and modifications.
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| Free Research Field |
分子遺伝学
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