Molecular mechanism of regulation of mRNA stability and translation by RNA-binding proteins

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570192
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: University of Tsukuba
• Principal Investigator: IRIE KENJI 筑波大学, 医学医療系, 教授 (90232628)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ＲＮＡ / 出芽酵母 / 細胞極性 / ＲＮＡ結合タンパク質 / 細胞壁 / ポリＡ分解酵素

Outline of Final Research Achievements

In this study, we show that PBP1 genetically interacts with CCR4 and KHD1, which encode a cytoplasmic deadenylase and an RNA binding protein, respectively. Ccr4 and Khd1 modulate a signal from Rho1 in the cell wall integrity pathway by regulating the expression of RhoGEF and RhoGAP, and the double deletion of CCR4 and KHD1 confers severe growth defect displaying cell lysis. We found that the pbp1Δ mutation suppressed the growth defect caused by the ccr4Δ khd1Δ mutation. Then we screened novel Pbp1-interacting factors, and found that Pbp1 interacts with ribosomal proteins, Rpl12a and Rpl12b. Similarly to the pbp1Δ mutation, the rpl12aΔ and rpl12bΔ mutation also suppressed the growth defect caused by the ccr4Δ khd1Δ mutation. Our results suggest that Pbp1 is involved in the Ccr4 and Khd1-mediated regulation of cell growth through the association with Rpl12a and Rpl12b.

Free Research Field

分子細胞生物学