2014 Fiscal Year Final Research Report
Posttranscriptional regulation of clock genes by ABC protein
| Project/Area Number |
24570199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ショウジョウバエ / 概日リズム / 時計遺伝子 / ABCタンパク質 |
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| Outline of Final Research Achievements |
CG9281, belonging to the ATP-binding cassette family, was identified as a new clock related gene through the clock cell specific RNAi screening in Drosophila. Both the knockdown flies and the transposon insertional mutant of CG9281 showed a longer circadian period and altered mRNA levels in clock genes. Casein kinase 2 (CK2), which phosphorylates the clock proteins PER/TIM, was co-immunoprecipitated with CG9281 although the behavior analysis using CK2 mutants demonstrated that CG9281 affects circadian clock through the different pathway from CK2. Series of luciferase assays and behavior analyses imply that dominant negative mutants, which lacks the function of the second ATP-binding domain of CG9281, enhances the negative auto-feedback effect of PER/TIM, presumably through a post-transcriptional effect.
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| Free Research Field |
時間生物学
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