Characterization of an arresting-like protein involved in the internalization of syntaxin during meiosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 24570219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka City University
• Principal Investigator: Nakamura Taro 大阪市立大学, 大学院理学研究科, 教授 (30291082)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 膜輸送 / シンタキシン / アレスチン / 酵母 / 胞子形成

Outline of Final Research Achievements

We conducted live imaging of endocytosis during meiosis by fluorescence microscopy and demonstrated that Mug170 localizes to the specific region on the plasma membrane of zygote. We also observed dynamic behavior of Psy1 during meiosis and determined the lysine residue required for its internalization. No HECT-type ubiquiin ligase mutants showed defects in internalization of Psy1. Moreover, we identified 20 genes involved in Psy1 internalization, which encode ubiquitin ligase, cyclin, and microtubule-related proteins.

Free Research Field

分子細胞生物学