Role of genistein-induced multidrug efflux pump of Bradyrhizobium japonicum on symbiosis with soybean

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: OHWADA Takuji 帯広畜産大学, 畜産学部, 教授 (90211804)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ダイズ根粒菌 / 多剤排出ポンプ / ゲニステイン / 相利共生

Outline of Final Research Achievements

One genomic region, which is induced by genistein, was found in Bradyrhizobium japonicum and named BjG30. The induction level reached maximum at 15 min after the addition of genistein and the induction profile was different from that of symbiosis genes (nod genes). The BjG30 composed of multidrug efflux pump, polyhydroxybutyrate metabolism, and transcriptional regulatory genes, and played an important role in symbiosis with soybean for the nodule formation and nitrogen fixation. In addition, the results of global gene expression analyses showed that the efflux of genistein would functionally regulate the expression levels of symbiosis genes.

Free Research Field

応用微生物学