Analysis of oscillatory calcium signaling and its decoding machinery in the early secretory pathway

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: SHIBATA Hideki 名古屋大学, 生命農学研究科, 准教授 (30314470)
• Co-Investigator(Renkei-kenkyūsha): MAKI Masatoshi 名古屋大学, 大学院生命農学研究科, 教授 (40183610)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: シグナル伝達 / カルシウム結合タンパク質 / COPII小胞 / 小胞体 / アネキシン

Outline of Final Research Achievements

In this study, we focused on calcium-binding proteins that are recruited to the specialized region of endoplasmic reticulum, called ER exit site (ERES) and investigated their roles in the early secretory pathway in response to calcium oscillation. The penta-EF-hand protein ALG-2 is a calcium-dependent interacting protein for Sec31A, an outer coat component of COPII, and recruits a calcium-dependent phospholipid-binding protein annexin A11 (AnxA11) to the ERES. The siRNA-mediated knockdown experiments revealed that both ALG-2 and AnxA11 stabilize Sec31A at the ERES and regulate intracellular distribution of the ERES. In the knockdown cells, the transport of GFP-fused vesicular stomatitis virus tsO45 glycoprotein from the ER to the Golgi was accelerated. These results suggest that AnxA11 regulates architectural and functional features of the ERES by coordinating with ALG-2 in response to calcium oscillation.

Free Research Field

応用生物化学