Elucidation of cellular functions by myristoylation-dependent protein-protein interaction

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto Gakuen University
• Principal Investigator: MATSUBARA Mamoru 京都学園大学, バイオ環境学部, 教授 (90288481)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ミリスチル化 / ミリストイル化 / 脂質修飾 / シグナル伝達 / 蛋白質間相互作用 / リン酸化 / 転写抑制因子

Outline of Final Research Achievements

A variety of oncogene and signal transduction proteins are known to be myristoylated. Although the role of myristoylation in protein-lipid interaction is well established, the involvement of myristoylation in protein-protein interaction is less well understood.
To elucidate the myristoylation-dependent protein-protein interaction, we detected the binding proteins of NAP22 in a miyristoyaltion-dependent manner. The binding proteins included the nuclear proteins such as HMGB1. Protein-protein interactions of HMGB1 and myristoylated NAP-22 were observed in cytosol and nuclear regions of various mammalian cells. These results suggest that myristoylation-dependent protein-protein interaction might be very significant to cellular function.

Free Research Field

蛋白質科学、細胞生物学、分子生物学、生化学