Application and genetical investigation of novel cellulase produced by Hirondellea gigas

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: Kobayashi Hideki 国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 主任研究員 (40399564)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: セルラーゼ / ヨコエビ / 深海 / RNA

Outline of Final Research Achievements

Hirondellea gigas in the Challenger Deep has novel cellulase, however, its application is difficult because of Convention on Biological Diversity. Therefore, I researched amphipods in the Izu-Ogasawara Trench, and found that they have the cellulase same properties as H. gigas. I extracted total RNA from the amphipod from the Izu-Ogasawara Trench, and obtained RNA sequence data using Next Generation Sequencer. I found seven sequences related with cellulase in the RNA sequence data. I tried PCR amplification of cellulase genes from cDNA, however, I cannot obtain the same sequence as seven cellulase candidates. Therefore, there would be some mistakes in the assembling of sequence data.

Free Research Field

分子生物学