Screening for anti-aging food that regulates intestinal function and clarification of its molecular basis

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: Yoshinori Katakura 九州大学, (連合)農学研究科(研究院), 准教授 (50264106)
• Research Collaborator: FUJII Kaoru ; HARADA Gakuro ; YAMASHITA Shuntaro ; KADOOKA Keishi
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: アンチエイジング / 乳酸菌 / SIRT1 / がん抑制

Outline of Final Research Achievements

We used human colon cancer cell line, Caco-2 cells, and established recombinant Caco-2 cells expressing EGFP under the control of human SIRT1 promoter. We monitored the changes in EGFP fluorescence after adding lactic acid bacteria by using flow cytometer, and identified T2102 strain as positive lactic acid bacteria. T2102 activated SIRT1, deacetylated and destabilized β-catenin. T2102 repressed hTERT expression and induced cellular senescence in Caco-2 cells. Furthermore, tumor suppressing activity of T2102 was evidenced by anchorage-independent growth and tumorigenicity.

Free Research Field

食品機能学