Changes in behevior and cell membrane structure in the central nerous sysytem of apoE- and ABC A1-deficient mice

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kinjo Gakuin University
• Principal Investigator: TSUSHIMA Hiromi 金城学院大学, 薬学部, 教授 (10080079)
• Co-Investigator(Kenkyū-buntansha): DOHMAE Sumiko 名古屋市立大学, 医学研究科, 准教授 (70227700)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 行動解析 / apo Eノックアウトマウス / ABCA1 ノックアウトマウス / カベオリン

Outline of Final Research Achievements

We investigated functions of the central nervous system (CNS) and caveolin distribution/expression using rats fed high-fat diet, apoE knockout (KO) mice and ABC A1 KO mice showing high and low concentration of cholesterol in the plasma, respectively. The high-soybean oil intake induced abnormal regulation of body temperature in the CNS, increase in n-6/n-3 ratio of fatty acid composition of the hypothalamus, decrease in caveolin mRNA expression. On the other hand, apoE KO mice showed spatial cognitive dysfunction and hypoalgesia, and in ABCA1 KO mice, the opposite behavior was observed. Caveolin mRNA expression in the hypothalamus of ABC A1 KO mice was increased, however, the protein did not increase. ABCA7 KO mice did not change expression of caveolin protein.

Free Research Field

薬理学