2014 Fiscal Year Final Research Report
Physiological Significance of Redistribution of Intermediate Filaments in the Mammalian Oocytes and Its Signal Transduction Pathways
| Project/Area Number |
24580403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 卵母細胞 / 卵成熟 / 中間径線維 / ニューロフィラメント / GFAP / ネスチン / デスミン / 哺乳動物 |
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| Outline of Final Research Achievements |
Function and redistribution of intermediate filaments in the mammalian oocytes during maturation were examined by confocal laser scanning microscopy. In porcine oocytes, distributional changes of keratin and vimentin were not clear, but those of neurofilaments (NF), GFAP and nestin were clarified during oocyte maturation. Interestingly, it is suggested that NF, GFAP and nestin may be included in the movement of lipid droplets in the ooplasm and that NF may function in chromosomal segregation at the second meiotic division. Furthermore, desmin may participate in anchoring the meiotic apparatus to the cortical region of oocytes, collaborating with actin microfilaments. Effects of the cell-cycle regulating factors and Rho kinase on the cytoskeletal architecture of the intermediate filaments were also discussed.
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| Free Research Field |
家畜繁殖学
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