Investigation of pathogenic mechanisms of new pathogenic factor NetB from Clostridium perfringens that causes avian necrotic entelitis.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580449
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: MUKAMOTO Masafumi 大阪府立大学, 生命環境科学研究科(系), 教授 (80231629)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細菌毒素 / 腸炎 / ニワトリ / 感染症 / ウエルシュ菌 / NetB

Outline of Final Research Achievements

It was clear that strains of possessing netb in C. perfringens type A were widely distributed in Japan and were infectious in healthy chickens. Though netb exist on a conjugative plasmid, netb was not easily transferred among cells and strains possessing netb that are rampant throughout the same areas were single or a small number. NetB is a pore forming toxin that possessed high specific pathogenic activities against the order of pheasants containing chickens. NetB was bound with non-raft regions on target cell membranes without participation of cholesterol. NetB polymerized among neighboring monomer molecules formed oligomer formation. In this study I made a part of molecular movement from binding to cells to pore formation of NetB clear.

Free Research Field

獣医衛生学、獣医感染症学