2014 Fiscal Year Final Research Report
Studies on a novel protein complex involved in RNA metabolism during ribosome biogenesis and its regulation by a molecular chaperone
| Project/Area Number |
24590101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIDA Yo-ichi 明治薬科大学, 薬学部, 講師 (90510454)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | リボソーム生合成 / リボソームRNA / シャペロン |
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| Outline of Final Research Achievements |
A chaperone-like AAA-ATPase NVL2 acts on a pre-ribosomal particle during ribosome biogenesis in eukaryotic cells through interaction with an RNA helicase DOB1, and it participates in a remodeling of the molecular complex. In this study, we have analyzed an rRNA metabolic complex consisting of DOB1, RNA exosome, and other associating factors, which could be a possible target of NVL2.
As a result, presence of TRAMP-like complex consisting of DOB1, a poly(A) polymerase PAPD5, and an RNA-binding protein ZCCHC7 was confirmed in mammalian cells. Furthermore, we have shown a possibility that this complex is functions in the 3’-polyadenylation of nuclear RNAs including the rRNA precursors.
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| Free Research Field |
細胞生物学
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